Fig. 3
From: Overexpression of CYP11A1 recovers cell cycle distribution in renal cell carcinoma Caki-1
CYP11A1 promotes apoptosis by inhibition of MAPK signaling. A ROS levels determined by the concentration of hydrogen peroxide generated under oxidative stress conditions. B Apoptosis of HEK293 and Caki-1 cells transfected with CYP11A1 for 24 h detected using Annexin V-PI double staining followed by flow cytometry. C The percentage of dead cells in each group. D Western blotting of p–C-Raf, ERK, p-ERK, JNK, and p38 expression. E protein expression levels were normalized to GAPDH