Fig. 3From: Cirsiliol regulates mitophagy in colon cancer cells via STAT3 signalingCirsiliol decreases the mitochondrial membrane potential, promotes the generation of ROS, and disrupts mitochondria network morphology. HCT116 and SW480 cells were treated with cirsiliol for 24 h. A, B Mitochondrial membrane potential of HCT116 (A) and SW480 cells (B) was determined by JC-1 staining. Representative fluorescence images of JC-1 aggregate (red) and JC-1 monomer (green) are shown in the Figure. Scale bar: 50 μm. C, D Quantification of mitochondrial membrane potential (JC-1 aggregate/monomer ratio) for HCT116 (C) and SW480 cells (D). E H2DCFDA staining was performed to detect ROS production for HCT116 and SW480 cells. Scale bar: 50 μm. F Quantification of H2DCFDA fluorescence intensity. G We performed Mito-Tracker Red staining to detect the mitochondria network morphology of SW480 cells. Representative images were presented in the figure. Scale bar: 10 μm. H–J MiNA was used to analyze the morphology of the mitochondrial network, including percentage of network mitochondria (H), mean branch length (I), and mitochondrial footprint (J). n = 5 (A-F), and 10 (G–J) per group. Data represent means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs cirsiliol 0 μMBack to article page