Fig. 7

The expression profile of survival-related IRCGS in HCC patients and their significant influence on the progression of HCC. A–C The mRNA-expressed level indicated that RHOA and ZC3HAV1 were up-regulated in tumor samples than in normal liver tissues. However, the VAV1 showed the reverse tendency. D, F The immunohistochemistry (IHC) was performed to detect the staining intensity and area in tumor samples and adjacent liver tissues from eight clinical patients with HCC. (D) indicated the representative staining images of VAV1, RHOA, and ZC3HAV1, and (F) was the statistical result. E Western blotting was used to determine the protein expression of key IRCGS in human HCC cells and immortalized liver cells. G–I The gene depletion assay via CRISPR system showed the gene effect score of key IRCGS in various HCC cell lines from DepMap database. A lower gene effect score indicated a higher possibility that the target gene is essential in a certain cell line, and the 0 score means that the gene is not necessary, correspondingly, -1 is the median value of all pan-essential genes. J–L The difference in EMT scores in the low and high expression of VAV1, RHOA, and ZC3HAV1 in HCC datasets from TCGA, ICGC, and GSE14520 were compared, respectively. All *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. EMT: epithelial-mesenchymal transition