Fig. 1

MDZ inhibited proliferation and invasion in HCC cell lines. A, B The cell viability histograms of the HCC-LM3 and Hep-3B cell lines were drawn after treatment with midazolam at increasing concentrations (0, 50, 75, 100, 150, or 200 µM) based on CCK-8 assays. C, D EdU assays were performed to assess the proliferation of HCC-LM3 and Hep-3B cells cultured with 75 and 150 µM MDZ for 24 h. Then, statistical analysis was performed on the EdU( +) proliferating cells. Cells were treated with stroke-physiological saline solution (SPSS) as the control. E, F Transwell experiments were used to assess the invasion of HCC cells incubated with MDZ. The left panel shows HCC-LM3 and Hep-3B cell invasion after MDZ treatment. The right panel shows the counts of invading cells. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001