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Fig. 3 | Cancer Cell International

Fig. 3

From: Exosomal LINC00460/miR-503-5p/ANLN positive feedback loop aggravates pancreatic cancer progression through regulating T cell–mediated cytotoxicity and PD-1 checkpoint

Fig. 3

MiR-503-5p is the direct target of LINC00460. A The predicted targets of LINC00460 from databases (StarBase and TCGA) were exhibited by Venn diagram. B Five miRNAs selected from databases were detected by RIP assay in PANC-1 cells. **P < 0.01 vs. the MS2 group. C The expression of miR-503-5p in PAAD tissues and normal tissues was analyzed in TCGA. *P < 0.05. D The predicted binding site between LINC00460 and miR-503-5p. E The luciferase activity in PANC-1 cells transfected with pGL3-LINC00460 WT/pGL3-LINC00460 MUT and miR-503-5p mimics/NC was determined by DLR assay. **P < 0.01 vs. the miR-NC group. F The expression of miR-503-5p after transfection of sh-LINC00460-1/NC into PANC-1 cells was detected by qRT-PCR. **P < 0.01 vs. the sh-NC group. G The expression of miR-503-5p in tumor tissues (n = 73) and adjacent tissues (n = 73) was detected by qRT-PCR. P < 0.01. H The correlation between LINC00460 and miR-503-5p. P < 0.01, r = −0.4115. I The expression of miR-503-5p in H6C7 cells and PC cell lines (ASPC-1, BxPC-3, SW1990, PANC-1 and Mia-PaCa-2) was detected by qRT-PCR. **P < 0.01 vs. the H6C7 cells group

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Cancer Cell International

ISSN: 1475-2867

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