Skip to main content
Fig. 6 | Cancer Cell International

Fig. 6

From: Exosomal LINC00460/miR-503-5p/ANLN positive feedback loop aggravates pancreatic cancer progression through regulating T cell–mediated cytotoxicity and PD-1 checkpoint

Fig. 6

LINC00460 knockdown inhibits the proliferation, migration and invasion, and promotes the apoptosis and G0/G1 phase arrest of PANC-1 cells by regulating miR-503-5p/ANLN. A The protein level of ANLN after transfection of pcDNA-ANLN/NC into PANC-1 cells was measured by western blot assay. **P < 0.01 vs. the pcDNA-NC group. B The expression of miR-503-5p after transfection of miR-503-5p inhibitor/miR-NC into PANC-1 cells was measured by qRT-PCR. **P < 0.01 vs. the miR-NC group. C The viability (OD450) of PANC-1 cells was measured by MTT assay. D The apoptosis of PANC-1 cells was analyzed by flow cytometry assay. E The cell cycle of PANC-1 cells was analyzed by flow cytometry assay. F The migration ability of PANC-1 cells was measured by transwell assay. G The invasion ability of P PANC-1 cells was measured by transwell assay. H The protein levels of E-cadherin and N-cadherin were determined by Western blot. I The proliferation of PANC-1 cells was determined by EdU assay (200 ×). **P < 0.01 vs. the sh-NC group, ##P < 0.01 vs. the sh-LINC00460 group

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us