Fig. 2
Vaccination with hAEC conferred protection and prolonged survival in murine models of colon cancer and melanoma but not in breast cancer. The top panel shows vaccination and tumor challenge scheme. 1 × 106 hAEC were injected subcutaneously into BALB/c (n = 6) and C57BL/6 mice (n = 10) in phosphate-buffered saline three times at 1 week interval. The control mice received only PBS. Mice were challenged seven days after the last vaccination with 5 × 105 CT26, 105 4T1 (BALB/c mice) or 105 B16F10 cells (C57BL/6 mice) in the same side as the vaccination side. Tumor volumes were regularly monitored and calculated by measuring tumor dimensions (L × W) with digital calipers. In the case of survival rate, mice were considered dead when tumor surface area exceeded 225 mm2. Error bars denote mean ± SEM. Asterisks (*) indicate statistical significant differences (**p < 0.01, ****p < 0.0001). hAEC: human amniotic epithelial cells, PBS: Phosphate-buffered saline