Fig. 3
From: RBM10 regulates alternative splicing of lncRNA Neat1 to inhibit the invasion and metastasis of NSCLC
RBM10 promotes PTEN expression and inhibits the phosphorylation of PI3K/AKT/mTOR signaling pathway. A PTEN protein expression in oe-RBM10 and sh-RBM10 NSCLC cells by Western blot analysis, normalized to GAPDH. B Immunofluorescence qualitative analysis of PTEN protein expression (100 ×). The blue fluorescence represents the nucleus, the red fluorescence represents the PTEN protein, and the red fluorescence is mainly located in the cytoplasm. C The phosphorylation of PI3K, AKT, and mTOR in oe-RBM10 and sh-RBM10 NSCLC cells by Western blotting analysis, normalized to GAPDH. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data are means ± SD