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Fig. 2 | Cancer Cell International

Fig. 2

From: HER2 amplification by next-generation sequencing to identify HER2-positive invasive breast cancer with negative HER2 immunohistochemistry

Fig. 2

HER2 amplification by NGS in a breast carcinoma with a negative HER2 IHC result. A, B Microscopy image of HER2 IHC 1 + in a surgical specimen. A is hematoxylin and eosin (H & E) staining and B is HER2 IHC staining. C Amplification of all probes of the HER2 gene by next-generation sequencing based on Log2-R ratio (> 1.3). D HER2 amplification by FISH. Dual-color HER2 FISH revealed ratio of the HER2 to D17Z1 (a centromere control probe) = 4.0, average number of HER2 signals per nucleus = 8.4, and average number of CEP17 signals per nucleus = 2.1

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Cancer Cell International

ISSN: 1475-2867

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