Fig. 2

ADMA Drives Macrophages Towards an M2-like subtype. RAW264.7 cells were treat-ed with DPBS (control) or ADMA (5 mM) for 3 days. RNA was collected and expression of M1- and M2-related genes was analyzed. Many M2 related markers were strikingly induced, in-cluding ARG (A), CD163 (B) and CD206 (C) while the M1 related markers NOS (D) and TNFα (E) were slightly or not impacted by ADMA. However, some other M2 related markers were downregulated such as IL10 (F) and Dectin-1 (G). Additionally, the chemotactic receptor CCR2 was also downregulated by ADMA (H). The most dramatically upregulated M2 markers (CD163 and CD206) by RNA expression were validated at the protein level by flow cytometry (I). Data are presented as mean ± SD. (*p < 0.05, *** p<0.001, ****p<0.0001), N=4 for all groups