Fig. 6

All Normal, Bulk Tumor Cells and CSCs Made ADMA but the Expression of PRMT1 and Jmjd6 was Different. All cell lines including mouse normal-like epithelial HC11, mouse breast cancer EMT6 and 4T1 cells were capable of generating and secreting ADMA into conditioned medium (A). The same was also observed using the stem cell cultures (B). RNA expression analyses showed that normal- like HC11 cells had high expression of the arginine methylating enzyme, PRMT1 (C) but also very high expression of the arginine demethylating enzyme, jmjd6 (D). The tumor cells, EMT6 and 4T1, by contrast, expressed lower levels of PRMT1 (C) and even lower relative levels of jmjd6 (D). ADMA concentration was expressed as ng/mL (left Y axis) or µM (right Y axis). Data are presented as individual replicates or mean ± SD. (*p < 0.05, ** p < 0.01, ****p < 0.0001), N = 5 for all ELISA (A, B, E) and N = 4 for the real-time PCR analyses (C, D)