Fig. 4

Knockdown of ALKBH5 inhibits the angiogenesis of lung cancer in vitro and in vivo. A, B The expression of VEGFA in A549 and H1975 cells when ALKBH5 was knocked down was detected with qRT‒PCR and western blotting. C, D HUVECs were cultured with conditioned medium from A549 or H1975 cells transfected with si-ALKBH5 or NC, and the tube formation was quantified. E The blood vessels sprouting from subintestinal vessels in zebrafish larvae were analyzed at 48 h after injection of A549 cells with si-ALKBH5 or NC transfection. The red signals indicate the cancer cells labeled CM-DiI. The green signals indicate the blood vessels that expressed EGFP. The white arrows indicate the new blood vessels that sprouted from subintestinal vessels in zebrafish larvae. NC: n = 12, si-ALKBH5: n = 12. Scale bar: 100 μm. *: P < 0.05, **: P < 0.01, ***: P < 0.001