Fig. 5

Knockdown of ALKBH5 decreases the stability of PVT1 but not VEGFA mRNA in lung cancer cells. A m6A modification sites of VEGFA mRNA were predicted by SRAMP. B The stability of VEGFA over time in A549 cells transfected with si-ALKBH5 was measured by qRT‒PCR relative to time point 0. C qRT‒PCR was used to detect the expression of PVT1 in A549 and H1975 cells after silencing ALKBH5. D Kaplan‒Meier analysis was used to analyze the correlation between PVT1 expression levels and overall survival of patients with lung cancer from the TCGA database. E m6A modification sites of PVT1 were predicted by SRAMP. F RIP experiments were performed in A549 cells and the coprecipitated RNA was subjected to qRT-PCR for PVT1. Expression levels of PVT1 are as fold enrichment in ALKBH5 antibody relative to IgG immunoprecipitates. G The stability of PVT1 over time in A549 cells transfected with si-ALKBH5 or pcDNA3.1-ALKBH5 was measured by qRT‒PCR relative to time point 0. *: P < 0.05, **: P < 0.01, ***: P < 0.001