Fig. 6

PVT1 promotes the proliferation, metastasis and angiogenesis of lung cancer cells. A–C CCK-8 assays were performed to evaluate the proliferation of lung cancer cells when PVT1 was knocked down or overexpressed. D-F Cell migration was evaluated by transwell assays when PVT1 was knocked down or overexpressed in lung cancer cells. G, H Statistical analysis of proliferation and metastasis in A549 cells after silencing PVT1 in zebrafish xenografts. NC: n = 13, si-PVT1: n = 13. I, J VEGFA expression in A549 and H1975 cells with PVT1 knockdown was measured by qRT‒PCR and western blotting. K HUVECs were cultured with conditioned medium from A549 cells transfected with si-PVT1 or NC, and the tube formation was quantified. *: P < 0.05, **: P < 0.01, ***: P < 0.001