Fig. 7

PVT1 overexpression partially restores the proliferation, migration and angiogenesis of lung cancer following suppression by ALKBH5 knockdown. A PVT1-regulated genes were examined by qRT-PCR in A549 cells cotransfected with si-ALKBH5 or NC and the pcDNA3.1 or pcDNA3.1-PVT1 plasmid. B CCK-8 assays were performed to assess proliferation in A549 cells cotransfected with si-ALKBH5 or NC and the pcDNA3.1 or pcDNA3.1-PVT1 plasmid. C Transwell assays were performed to assess the migration of A549 cells cotransfected with si-ALKBH5 or NC and the pcDNA3.1 or pcDNA3.1-PVT1 plasmid. D HUVECs were treated with conditioned medium from A549 cells cotransfected with si-ALKBH5 or NC and the pcDNA3.1 or pcDNA3.1-PVT1 plasmid, and the tube formation was quantified. *: P < 0.05, **: P < 0.01, ***: P < 0.001