Table 1 Primer sequences for qRT‒PCR
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
GAPDH | GGGAGCCAAAAGGGTCAT | GAGTCCTTCCACGATACCAA |
PVT1 | TTGGCACATACAGCCATCAT | CAGTAAAAGGGGAACACCA |
VEGFA | CTGTCTTGGGTGCATTGGAG | ACCAGGGTCTCGATTGGATG |
ALKBH5 | CGGCGAAGGCTACACTTACG | CCACCAGCTTTTGGATCACCA |
CCND1 | CCCTCGGTGTCCTACTTCAAATGT | GGAAGCGGTCCAGGTAGTTCAT |
CDK1 | TTTTCAGAGCTTTGGGCACT | CCATTTTGCCAGAAATTCGT |
MMP2 | CTGCGGTTTTCTCGAATCCATG | GTCCTTACCGTCAAAGGGGTATCC |
MMP9 | GAGGCGCTCATGTACCCTATGTAC | GTTCAGGGCGAGGACCATAGAG |
Vimantin | AAGTTTGCTGACCTCTCTGAGGCT | CTTCCATTTCACGCATCTGGCGTT |