Fig. 4

CCR2 and CXCR2 antagonists reduce the recruitment of TAMs/TANs in the primary HCC model. Sprague Dawley rats were treated with diethylnitrosamine for 14 weeks, then INCB334 and SCH527123 were administered via intraperitoneal injection. After 14 weeks, the rats were sacrificed and the livers were removed for immunohistochemical detection of CD68 and MPO. CD68 is a specific marker for TAMs while MPO is a specific marker for TANs. The expression levels of CD68 and MPO in the stained sections was assessed by IOD. A, C Representative images of CD68 and MPO immunostaining in livers from the indicated groups. Asterisks indicate positive staining. Scale bar, 200 μm. B Comparison of CD68 levels, based on IOD, of the indicated groups. D Comparison of MPO levels, based on IOD, of the indicated groups. Data are presented as mean ± SD. ns not statistically significant, *p < 0.05, **p < 0.01