Fig. 2

Effect of CYLD down-regulation on intracellular signaling pathways in OSCC cells. A Comprehensive changes in protein expression by CYLD knockdown were assessed by proteome analysis. B KEGG database analysis of cell signaling pathways activated by CYLD knockdown. C Phosphorylation of Akt and ERK was assessed by immunoblotting. Cells were transfected with the indicated siRNAs and then incubated for 72 h before harvesting. Cell lysate was immunoblotted with antibodies against the indicated proteins. D Cells were treated with LY294002 (Akt Inhibitor, 20 µM), PD98059 (ERK Inhibitor, 20 µM), combination, and cisplatin (8 µg/mL). Cells survival rate are assessed after 72 h. Values are means ± S.D. of triplicate samples. **p < 0.01 in Tukey–Kramer method