Fig. 6
From: Extracellular matrix stiffness mediates radiosensitivity in a 3D nasopharyngeal carcinoma model
Proportion of tumor stem cells increased in 3D culture system A The formation of soft agar colonies was significantly different in each group on the 1st, 6th, and 12th day (scale bar 100 μm). B After fixation and staining, the well was photographed and the colonies were observed under the microscope (scale bar 100 μm). C The number of soft agar colonies in each group (n = 3, *P < 0.05). The number of tumor stem cells in the two 3D groups was significantly increased. D Western Blot analysis of SOX-2 and CD44 expression in each group. The expression of SOX-2 and CD44 were enhanced compared with 2D group