Fig. 3

TRAP1 expression is associated to a less energetic metabolism. A, B Extra cellular acidification rates (ECAR) in HeLa cells following induction of shGFP/shTRAP1 for 72 h (A) or GFP/TRAP1-GFP for 48 h (B), assayed by the SeaHorse platform as described under “Metabolic analyses” section. Glycolysis, resting ECAR; Glycolytic Capacity, ECAR measured after the addition of oligomycin and FCCP and refers to the maximal glycolytic activity with the OxPhos inhibited; Glycolytic Reserve, difference between ECAR measured in the presence of oligomycin and under resting conditions. The ECAR values were corrected for the residual ECAR measured after the addition of the 2DG glycolytic inhibitor. Before metabolic assay procedures, cells have been cultured in standard culture medium (4.5 g/L glucose) or in low glucose (1 g/L) for 18 h (GD: glucose deprivation). C, D Viability assays performed in HeLa cells following induction of shGFP/shTRAP1 (72 h) or GFP/TRAP1-GFP (24 h), upon 48 h of complete glucose (C) or glutamine deprivation (D). The two-tailed p value represents the statistical significance based on the Student’s t-test. E, F Energy map obtained by plotting OCR vs ECAR profiles of shGFP, shTRAP1, GFP and TRAP1-GFP HeLa cells