Fig. 6

Platinum-resistant HGSOC cells display high complex III expression/activity. A Total lysates obtained from platinum-sensitive PEA1 and matched platinum-resistant PEA2 cells or from platinum-sensitive PEO1 and matched platinum-resistant PEO4 cells were separated by SDS-PAGE and immunoblotted with the indicated antibodies. Images are representative of four independent experiments. Bar graphs below each image represent densitometric quantification of bands, expressed as mean ± SEM (n = 4). Numbers above bars indicate the statistical significance (p value) obtained by the Student’s t-test (significant p values are highlighted in red). B Viability assays performed in cisplatin-sensitive PEA1 and cisplatin-resistant PEA2 cells following treatment with Antimycin A and Rotenone at increasing concentrations for 48 h. Data are expressed as mean ± SEM of four independent experiment, with technical triplicates each. Numbers indicate the statistical significance (p value) obtained by the Student’s t-test (significant results are highlighted in red). C BEC index analysis on PEO1 and PEO4 cells. Cells were harvested and total protein lysates were immunoblotted with anti-βF1ATPase, anti-HSP60 and anti-GAPDH antibodies. Immunoreactive bands were quantified by using ImageJ and BEC index was calculated by the formula F1ATPase/HSP60/ GAPDH (see Material and Methods section for details). Data are expressed as mean ± S.E.M. from three independent experiments. Numbers above bars indicate the statistical significance (p value), based on the Student’s t-test. D Representative image of PLA showing the interaction of TRAP1 with UQCRC2 or Rieske in PEA1 cells. Positive signals of interaction are shown as red dots, nuclei are stained with DAPI (blue). Negative control has been obtained by hybridizing cells with TRAP1 antibody only. Scale bar: 10 µm