Fig. 5

Pull-down of APC^954–1203 containing the 15R region. GST-APC^954–1203 was tested against the following MBP-tagged proteins: Lane 1: FOSL1^FL, Lane 2: FOSL1^40–282, Lane 3: TCF7^152–359, and Lane 4: MBP alone (negative control). All proteins expressed with MBP show a leaky expression of MBP as indicated by a red band of 40.3 kDa and are present in protein expression gels (Additional file 1: Fig. S1BD). The signal of MBP alone in all samples (lanes 1–3) represents the binding of MBP protein to the amylose resin. Samples FOSL1^FL, FOSL1^40–282, and TCF7^152–359 show fragmentation represented in the gel by multiple bands below the expected target protein. Arrow points to the expected molecular weight of the target protein/fragment (green signal GST; red signal MBP). Each pull-down experiment was conducted in triplicate