Fig. 1

Phenotypical and histological characterization of Lyve-1^−/− livers. Iron deposition in F4/80⁺ cells in Lyve-1^−/− livers. Increased hyaluronan and carbonic anhydrase 2 levels as well as decreased levels of factor 13a1 and factor 13b in plasma of Lyve-1^−/− mice. A Body weight (female, P = 0.3912, n = 12/group; male, P = 0.1662, n = 11/group) and liver/body weight ratio of Ctrl and Lyve-1^−/− mice (female, P = 0.2343, Mann-Whitney U-test, n = 6-7/group; male, P = 0.2225, n = 6/group). B Representative pictures of Ctrl and Lyve-1^−/− livers (female, n = 6/group). Scale bars: 1 cm. C Immunoblot of Lyve-1 in Ctrl and Lyve-1^−/− livers (female and male, n = 4/group). D Immunofluorescence stainings for Lyve-1 and Emcn in Ctrl and Lyve-1^−/− liver tissue (female, n = 6/group) and quantification of Lyve-1 (P = 0.005) and Emcn (P = 0.3905) in Ctrl and Lyve-1^−/− liver tissue (female, n = 6/group). Scale bars: 100 µm. E H&E and Sirius red stainings of Ctrl and Lyve-1^−/− livers (female, n = 5/group). Prussian blue stainings and immunohistochemistries for F4/80 with Prussian blue co-staining of Ctrl and Lyve-1^−/− livers (female, n = 6/group). Iron deposits quantified in Prussian blue stained Ctrl and Lyve-1^−/− liver slides (female, P = 0.0043, Mann-Whitney U-test, n = 5-6/group). Scale bars: 100 µm. Black arrows highlight iron deposition. F Hyaluronan concentration in plasma of Ctrl and Lyve-1^−/− mice (female, 12 weeks, P = 0.0008, n = 6/group) measured with Hyaluronan DuoSet ELISA (DY3614-05, R&D Systems, Minneapolis, MN, USA). G Proteomics of plasma from Lyve-1-KO and Ctrl mice were performed. A clustered heatmap with significantly regulated proteins is presented (q-values: CA2: 0.0153; F13b: 0.018; F13a: 0.028). Data information: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns not significant