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Fig. 6 | Cancer Cell International

Fig. 6

From: Evaluation of X-ray and carbon-ion beam irradiation with chemotherapy for the treatment of cervical adenocarcinoma cells in 2D and 3D cultures

Fig. 6

Involvement of CSCs in radiosensitivity. A Sphere culture protocols are shown. B The spheres during the 3-day incubation period. Sphere images were captured by microscopy. Scale bars: 500 μm. Sphere size and the equivalent circle diameter were calculated with NIS-Elements Advanced Research software. Cell viabilities were measured using Cell Titer-Glo 3D. C Cell populations for the CD49f and CD44v9, which are known markers of cervical CSC-like cells after irradiation, were obtained by flow cytometric analysis. D Representative FACS histograms showing increased CD49f and CD44v9 in irradiated 3D spheroid compared with 2D systems. E Percentages of cells expressing CD49f, CD44v9, or both are shown as a bar graph. Data are presented as the mean ± SD (n = 6), *p < 0.05, ** p < 0.01

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Cancer Cell International

ISSN: 1475-2867

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