Fig. 4
Repression of AGR2 in the primary cell lines. A Immunofluorescence images showing the expression of AGR2 following treatment using siRNA against AGR2 exons 2 and 5/6. AGR2 is shown in red and DAPI is shown in blue. All images were taken at 20x. B Barographs showing the mean cell densities within 3.9 mm2 for the primary cells transfected with the negative control or siRNA AGR2 oligos. Asterisks indicate significant Z-test differences at p < 0.05. Data were collected from three independent experiments. C Inhibition of AGR2 using a double-hit approach; i) A schematic diagram displaying the double-hit approach. ii) Immunofluorescence images showing the repression of AGR2 following two consecutive siRNA treatments using siRNA against AGR2 exon 2B. AGR2 is shown in red and DAPI in blue, and images were taken at 20 × magnification. iii) A barograph showing the survival fraction of cells transfected with Cy3, the negative control, or with two treatments of siRNA against AGR2 exon 2B. Two asterisks indicate significant T-test difference at p < 0.01. The error bars represent errors between counts for three independent experiments. iv) A barograph showing the mean cell densities within 3.9 mm2 for cells treated once or twice with negative control or siRNA against AGR2 exon 2B. The asterisk indicates a significant Z-test difference at p < 0.05. Data were collected from three independent experiments