Fig. 4
From: Targeting hyaluronic acid synthase-3 (HAS3) for the treatment of advanced renal cell carcinoma
Ectopic HAS3 expression attenuates the effect of SF and MU combination on RCC cell growth. A: Expression of Flag-tagged HAS3 protein in RCC cells. B, C: HAS3 protein expression in RCC cell transfectants following treatment with SF and MU combination for 48–60 h. The images are cropped for brevity. Uncropped images of the blots shown in panels A–C are provided in the Additional file 1 (Appendix). D, E: Measurement of HA levels in HAS3 transfectants’ CM using the HA test. F–I: As indicated, RCC cells were treated with SF, MU, or the combination. Viable cells were counted at 72 h. Dose-responsive curves generated by variable slope equation and actual data points are shown. Data in panels F–I: Mean ± SD (n = 6 to 8)