Fig. 5

Clonogenic survival and proliferation of HAS3 and HAS3 knockdown transfectants treated with the combination. A: Clonogenic survival of Caki-1 EV and HAS3 transfectants following treatment (as indicated). Crystal violet staining (left panel) and quantification (right panel) of the colonies stained on day 7. B: HAS3 protein expression in HAS3 shRNA transfectants of RCC cells. Actin is the loading control. The images are cropped for brevity. Uncropped images of the blots shown in panel B are provided in the Additional file 1 (Appendix) C, D: Control and HAS3 shRNA transfectants of RCC cells were treated with SF and viable cells were counted after 72 h. 5E: Viability of endothelial cells (HMEC-1, HULEC-5a) exposed to SF + MU in the presence or absence of HA was measured by MTT assay was performed at 72 h. Percent viability was calculated from untreated control (100%). Data in panels A and C–E: Mean ± SD (n = 3 to 8)