Fig. 4

Effects of OA treatment on stemness of H460 cells under glucose-deficient condition. A Transcription levels of Nanog and Oct4 in H460 cells incubated with OA in complete or glucose-deficient medium for one day. ^*P < 0.05 and ^***P < 0.001. Data indicate the mean ± SEM (n = 4). B Representative fluorescence image of BODIPY and Oct4 in H460 cells cultured in complete medium or glucose-deficient conditions with/without OA treatment for 1 day. Green, BODIPY; red, Oct4; blue, DAPI. 20 × magnification. Scale bar: 200 μm. C Staining for CD133 and CD44 in H460 cells treated with OA and CAY10566 in complete or glucose-deficient medium for 1 day. ^*P < 0.05 and ^***P < 0.001. Bar graph indicates the percentage of CD133 and/or CD44 positive. Data represent the mean ± SEM (n = 3). D Number of spheroids derived from H460 cells treated with OA and CAY10566 in complete or glucose-deficient medium for 7 days. Spheroids over 100 μm in diameter were counted using Image J. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001. Data represent the mean ± SEM (n = 5)