Fig. 10

Tumour burden and Kaplan Meier Survival curves of mice injected IP with TIMP-2 CRISPR/Cas9 edited gRNA1 and gRNA2 cells and their respective controls. A Tumour burden obtained from mice injected IP with TIMP-2 CRISPR/Cas9 edited gRNA1 and gRNA2 cell lines and their control counter parts. Five × 106 cells of each cell line were injected IP into each mouse, n = 10 mice/group. The graph demonstrates the average tumour burden obtained from the mice in each group. Values are mean ± SEM, significance was deduced using one-way ANOVA *p > 0.05; **p < 0.01 compared to Control and Parental cell lines. B Kaplan Meier survival curves of mice injected with the respective cell lines as indicated above. Median survival of mice treated is shown in the Table. Significance (p < 0.05; Chi square 7.818; df 3) between the survival curves were deduced by using a Gehan–Breslow–Wilcoxon test