Fig. 8

3D spheroid growth and mRNA expression of EMT-associated genes in CRISPR/Cas9 edited cell lines and their controls. The cell lines studied are: Parental (P), CRISPR control (C), gRNA2 (2) and gRNA1 (1). Spheroid formation on low attachment plates was monitored using light microscopy. A Images of spheroids every second day from day 0 to day 8. Photographs are of ×4 magnifications. B–D The mRNA expression of MMP-14, MMP-2, E-Cad, N-Cad, SNAIL and TGFβ1 deduced by qRT-PCR as described in “Methods”. Values are mean ± SEM and each bar graph represents n = 3 for each cell line collected in independent experiments. Significance was obtained using One-way ANOVA. *p < 0.05, when compared to the control and parental cell lines