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Fig. 2 | Cancer Cell International

Fig. 2

From: An antisense amido-bridged nucleic acid gapmer oligonucleotide targeting SRRM4 alters REST splicing and exhibits anti-tumor effects in small cell lung cancer and prostate cancer cells

Fig. 2

Reconstitution of REST by the exogenous vector in SCLC cells reduced cell viability. a SCLC cells (H146) were transfected with each Flag-tagged REST or sREST expression plasmid (5 or 15 μg/1.0 × 106 cells) by electroporation. Then, 24 h post-transfection, cells were collected and cell lysates were prepared. All samples were analyzed by SDS-PAGE followed by immunoblot analysis using anti-Flag antibody. n.s.; non-specific band b SCLC cells (H146) were transfected with each plasmid (5.0 μg /1.0 × 106 cells) by electroporation. After 72 h of transfection, cell viability was measured by CCK-8 assay. The assay was performed 3 times to confirm reproducibility. Data are present as the mean ± S.D. in triplicate. **P < 0.01

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Cancer Cell International

ISSN: 1475-2867

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