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Fig. 3 | Cancer Cell International

Fig. 3

From: An antisense amido-bridged nucleic acid gapmer oligonucleotide targeting SRRM4 alters REST splicing and exhibits anti-tumor effects in small cell lung cancer and prostate cancer cells

Fig. 3

REST splicing analysis and cell viability in SCLC cells transfected with REST SSO. a Scheme of REST splicing change by REST SSO. b SCLC cells (H146) were transfected with SSO (AmNA_[+ 21/ + 40]) or negative control oligonucleotide. Cells were collected after 48 h of transfection and total RNA was prepared. RT-PCR was performed using specific primers for REST and actin and PCR products were analyzed using polyacrylamide gels; c Cell viability was measured by CellTiter-Glo 3D assay every 24 h until 72 h of culturing. The assay was performed 3 times to confirm reproducibility. Data are presented as the mean ± SEM of three independent experiments (n = 3). ***P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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