Fig. 4

SRRM4 ASO decreased cell viability in SRRM4-expressing prostate cancer (PCa) cells. a PCa cells expressing SRRM4 (22Rv1 and VCaP) were transfected with various amounts of ASO (AmNA_7174) and negative control oligonucleotide (AmNA_26). Cells were collected after 24 h following transfection and RT-qPCR was performed using specific primers and 2^−ΔΔct values of SRRM4 mRNA expression were normalized with endogenous actin in non-treated cells (NT) as 1.0. The assay was performed multiple times with different amount of AmNA_7174 to confirm reproducibility. Data are presented as the mean ± SEM of three independent experiments (n = 3). ***P < 0.001. (t test); b After 72 h of transfection, cell viability was measured by CellTiter-Glo 3D assay. The assay was performed multiple times with different amount of AmNA_7174 to confirm reproducibility. Data are presented as the mean ± SEM of three independent experiments (n = 3). ***P < 0.001; c Cells were collected after 48 h following transfection. RT-PCR was performed using specific primers for REST and β-actin. PCR products were analyzed by performing electrophoresis using polyacrylamide gels. One of three independent experiments is shown