Fig. 4

TLR4 positive colorectal cancer cells demonstrated a higher chemotherapy resistance potential than TLR4 negative colorectal cancer cells. A–B TLR4 positive CRC cells were treated with 5-Fu or cisplatin for 24 and 48 h. At the end of the experiment, the cell viability in each group were examined by CCK-8 assay. TLR4 negative CRC cells were employed as control group; C–D the expression of TLR4 in TLR4 positive CRC cells were inhibited by siRNA or treated with aspirin and then the cells were exposed by 5-Fu or cisplatin for 24 and 48 h. At the end of the experiment, the cell viability in each group were examined by CCK-8 assay. TLR4 negative and positive CRC cells were employed as control group. (*P < 0.05; **P < 0.01; ***P < 0.001)