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Fig. 5 | Cancer Cell International

Fig. 5

From: Aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4

Fig. 5

The stemness of TLR4 positive colorectal cancer cells is stronger than TLR4 negative colorectal cancer cells. A Correlation analysis between TLR4 and stem cells markers (CD44 and CD166) mRNA level in CRC patients in the TCGA database; B cell clone formation assay were used to examine the clone formation potential in TLR4 positive and negative CRC cells; C realtime PCR was employed to determine the expression of stemness-related genes in CRC cells; D western blot was employed to determine the expression of stemness-related genes in CRC cells; E the expression of TLR4 in TLR4 positive CRC cells were inhibited by siRNA or treated with aspirin and then cell spheres formation assay were used to examine the clone formation potential in CRC cells; F the expression of TLR4 in TLR4 positive CRC cells were inhibited by siRNA or treated with aspirin and then realtime PCR was employed to determine the expression of stemness-related genes in CRC cells; G Western blot was employed to determine the expression of stemness-related genes in CRC cells; (**P < 0.01; ***P < 0.001)

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Cancer Cell International

ISSN: 1475-2867

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