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Fig. 3 | Cancer Cell International

Fig. 3

From: Heat shock protein 27 deficiency promotes ferrous ion absorption and enhances acyl-Coenzyme A synthetase long-chain family member 4 stability to promote glioblastoma cell ferroptosis

Fig. 3

Silencing HSP27 increases cellular ferrous ion-mediated ROS production. A, B Total iron levels and ferrous ion ratios before and after shHSP27 transfection of SHG140 and U251 cell lines were determined by colorimetric methods. C, D Colorimetric determination of ferrous ion expression in different groups after DFO treatment. E Expression of iron-associated proteins in control groups and after lentiviral transfection. F Observation of ROS fluorescence intensity before and after transfection under fluorescence microscopy, DCFH-DA staining, scale = 50 μm. G, H Flow cytometry quantitatively detects ROS before and after transfection. I, J The microplate reader detects changes in ROS in different treatment and control groups. The statistical analysis showed *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NSP > 0.05

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Cancer Cell International

ISSN: 1475-2867

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