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Fig. 5 | Cancer Cell International

Fig. 5

From: Heat shock protein 27 deficiency promotes ferrous ion absorption and enhances acyl-Coenzyme A synthetase long-chain family member 4 stability to promote glioblastoma cell ferroptosis

Fig. 5

The interaction between HSP27 and ACSL4 in glioma cells. A Immunoprecipitation followed by western blot analysis revealed the interaction between HSP27 and ACSL4 in glioma cells. B Immunofluorescent images of HSP27 and ACSL4 expression in glioma cells. The nuclei were stained with DAPI. Images were captured by a laser confocal microscope. C Correlation analysis of TCGA database HSP27 and ACSL4. D Western blot detects changes in ACSL4 after HSP27 silencing. E, F After cycloheximide (20 μmol/L) treatment, the ACSL4 stability test was performed. G SUMOylation analysis of ACSL4 in the control group and HSP27 in the silence group

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