Fig. 3

Inhibition of migratory and invasive in MCF-7 and 4T1 cells by THC treated. A MCF-7 and 4T1 cells were seeded on six-well plates. A single scratch was made after the cells grew about 90% confluence. After treatment of THC for 48 h, the cells were fixed and photographed. The lines indicate the area occupied by the initial scraping and migrated cells were quantified. B MCF-7 and 4T1 cells were seeded in the top chamber of transwell with serum-free medium and treated with vehicle or different concentrations of THC. After about 48 h, migrated cells were fixed, stained, photographed and quantified. C MCF-7 and 4T1 cells were treated with different doses of THC for 48 h, cells were harvested and western blot assay was carried out to detect the expression of MMP2, MMP9, TIMP2, E-cadherin, N-cadherin, Vimentin and β-actin served as loading control. Protein expression of MMP-2, MMP-9 and TIMP2 were quantified by the densitometry analysis using Image-Pro Plus and normalized against β-actin expression. Data are expressed as means ± SD from three experiments. (**P < 0.01, ***P < 0.001, compared to control)