Fig. 6

Effects of CYP1A1 knockdown on THC anti-breast cancer. A After synthesis of CYP1A1 knock-down plasmid, MCF-7 and 4T1 cancer cells were transfected with Lipo2000. Changes of mRNA expression levels of CYP1A1 in MCF-7 and 4T1 were mesasured. B The MCF-7 and 4T1 cancer cells were divided into four groups: Vehicle, THC (80 μM, sh-CYP1A1 and sh-CYP1A1 + THC(80 μM). The expression of CYP1A1, NF-κB associated protein and PD-L1 in each group were measured by RT–PCR and WB experiments. C CCK8 assay was used to analyze the effect of transfection of knock-down plasmid on the cytotoxicity of MCF-7 and 4T1 cancer cells. D The effect of transfection of knock-down plasmid on the clonogenesis of breast cancer cells was analyzed. E The effect of THC on apoptosis of breast cancer cells transfected with knock-down plasmid was using the Annexin V/PI dual-labeling technique, and determined by FCM. F The effect of THC on breast cancer cell metastasis after transfection with knock-down plasmid was analyzed by scratch assay. G Transwell assay was used to analyze the effect of THC on invasion of breast cancer cells after transfection with knock-down plasmid. Data are expressed as means ± SD from three experiments. (*P < 0.05; **P < 0.01; ***P < 0.001, compared to control; ^#P < 0.05, ^##P < 0.01, compared to THC)