Fig. 7

Effects of THC on tumor progression in MCF-7 tumor-bearing mice. A The MCF-7 tumor growth curves of different groups within the treatment process. B Variation of mice body weight of different groups within treatment progress. C Representative image of MCF-7 tumors of different groups at the termination of the experiment. D MCF-7 tumor weight of different treated groups at the termination of experiment. E Survival time of MCF-7 tumor-bearing mice of different treated groups at the termination of experiment. F The number of tumor stem cell-like cells (CD44+ CD24−) in tumor tissues of mice after THC administration. G Immunohistochemistry analysis of Ki-67, MMP-2, Cleaved caspase-3 and CYP1A1 of MCF-7 tumor sections after different treatments. H Expression levels of MMP-2, Cleaved caspase-3 and CYP1A1, NF-κB, IκBα and p-IκBα associated proteins of different treated MCF-7 tumor tissues were detected by western blot. I: H&E staining was used to analyze the pathological changes of the heart, liver, spleen, lung and kidney in each group after THC administration. Bars represent means ± SD of at least 3 independent experiments; *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the Vehicle group