Fig. 8

THC regulates tumor immune microenvironment by CYP1A1/NF-κB/PD-L1 axis to inhibit tumor growth in 4T1-bearing mice. A Bioluminescence images of mice bearing a pulmonary metastasis model of 4T1-luciferase cells after different treatments at determined time points (6, 10, or 14 days). B Quantitation of the bioluminescence signal in mice after different treatments at determined time points. C Survival time of 4T1-bearing mice after different treatments. D Number of nodules of lungs from different treated groups. E Lung tissues of lung metastases mice were taken for PCR test to detect the changes of MMP2, NF-κB, CYP1A1 and PD-L1 genes. F H&E staining analysis of lung tissues from different treated groups. G Lung weight of the 4T1-bearing mice after different treatments. H Changes of proportion of MDSCs in 4T1 tumors after different treatments. I Effect of THC on the number of M1 and M2 macrophages in tumor tissue. J Changes of proportion of CD4 + and CD8 + T cells in 4T1 tumors after different treatments. K The number of tumor stem cell-like cells (CD44+ CD24−) in tumor tissues of mice after THC administration. L Detection of tumor immune-related cytokines. M H&E staining was used to analyze the pathological changes of the heart, liver, spleen, lung and kidney in each group after THC administration. Bars represent means ± SD of at least 3 independent experiments; *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the Vehicle group