Fig. 2

Assessment of growth and GEM sensitivity in PCCs. PCCs grown in media containing 1.0 g/L or 4.5 g/L glucose were assessed for A viability using MTT assay, B proliferation using BrdU incorporation assay, and C. morphology by crystal violet staining. Next, PCCs incubated for 72 h with SFM or three different PSC-CMs (PSC-1, PSC-2, and HPaSteC) were investigated for changes in D proliferation and E sensitivity to GEM (10 µM) following 48 h treatment. Data presented as mean ± SEM of four replicates. For D, E, *p < 0.05, **p < 0.01 comparing PSC-CM with SFM. GEM, gemcitabine; PCC, pancreatic cancer cell; PSC-CM, pancreatic stellate cell-conditioned medium; SFM, serum-free DMEM