Fig. 3

Expression analysis of glucose metabolism pathway markers. A Schematic presentation of glucose to lactate conversion in PCCs. Cell lysates collected from B PCCs at baseline, and C PCCs incubated for 72 h with SFM or three different PSC-CMs (PSC-1, PSC-2, and HPaSteC) were analyzed by Western blot for expression of the indicated proteins. GAPDH and vinculin were used as loading controls. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GLUT1, glucose transporter 1; HK (1, 2), hexokinase 1, 2; MCT (1, 4), monocarboxylate transporters 1, 4; LDHA, lactate dehydrogenase A; PCC, pancreatic cancer cell; PDH, pyruvate dehydrogenase; PFK1, phosphofructokinase 1; PKM2, pyruvate kinase M2; PSC-CM, pancreatic stellate cell-conditioned medium; SFM, serum-free DMEM