Fig. 5

LDHA and MCT4 silencing promotes GEM sensitivity in PCCs. BxPC-3, Mia PaCa-2, and Panc-1 cells were transfected with NTC or siRNAs against LDHA and MCT4. Expression of LDHA and MCT4 was confirmed by A immunostaining and B western blot. Vinculin was used as loading control. Assessment of C proliferation, D extracellular lactate release, and E GEM sensitivity in PCCs transfected with NTC or siRNAs against LDHA and MCT4. Data presented as mean ± SEM of 4–6 replicates. For C and D, *p < 0.05, **p < 0.01 comparing NTC with LDHA or MCT4 transfected cells. For E, **p < 0.01 comparing control with GEM treated cells, and ^##p < 0.01 comparing NTC with LDHA or MCT4 transfected cells. NTC negative transfection control, LDHA lactate dehydrogenase A, MCT4 monocarboxylate transporter 4, PCC pancreatic cancer cell