Fig. 2

5-FU significantly inhibited cell viability and induced cell apoptosis in CRC cells. a–b Relative cell viability of CRC cells measured by the CCK-8 assay after treatment with 5-FU at various doses ranging from 1 to 100 μM for 24 or 48 h. c–g Cell apoptosis of CRC cells detected by flow cytometric analysis after treatment with 50 μM of 5-FU for 24 or 48 h. Data were expressed as the mean ± SD (n = 3) of three parallel experiments. ^*P < 0.05, ^**P < 0.01 vs control