Fig. 3

CAP showed the cytotoxic effect on the growth of CRC cells after pre-metabolized by HepG2 cells. a Schematic workflow to establish the model of pre-metabolism by HepG2 cells. First, HepG2 cells were treated with the indicated doses of CAP for 0, 24 or 48 h. Then the medium metabolized by HepG2 cells in each group was collected and added to CRC cells for the following treatments. b–f After HepG2 cells were treated with 200, 500 or 1000 μM of CAP for 0, 24 or 48 h, the medium of each group was collected and added to CRC cells for the following 48 h treatment. Relative cell viability of CRC cells was measured by the CCK-8 assay. Data were expressed as the mean ± SD (n = 3) of three parallel experiments