Fig. 4

CAP showed the cytotoxic effect on the growth of CRC cells under co-cultured with HepG2 cells. a Schematic workflow to establish the co-culture system of CRC and HepG2 cells with HepG2 cells in the upper compartment and CRC cells in the lower compartment. Then, the established co-culture system was incubated with the medium containing CAP. b–f Cell apoptosis of CRC cells under co-cultured with HepG2 cells detected by flow cytometric analysis after treatment with 500 μM of CAP for 24 or 48 h. g Apoptosis-related protein levels detected by western blotting in CRC cells after treatment with 500 μM of CAP under co-culture with HepG2 cells or not, and 50 μM of 5-FU for 48 h. Data were expressed as the mean ± SD (n = 3) of three parallel experiments. ^*P < 0.05, ^**P < 0.01 vs control. Abbreviations: DHFU, dihydrofluorouracil; FUPA, α-fluoro-β-ureidopropionic acid; FBAL, α-fluoro-β-alanine; FUMP, 5-fluorouridine 5′-monophosphate; FUDP, 5-fluorouridine 5′-diphosphate; FUTP, 5-fluorouridine 5′-triphosphate; FdUMP, 5-fluoro-2′-deoxyuridine 5′-monophosphate; FdUDP, 5-fluoro-2′-deoxyuridine 5′-diphosphate; FdUTP, 5-fluoro-2′-deoxyuridine; dTMP, 2′-deoxythymidine 5′-monophosphate; dUMP, 2′-deoxyurdine 5′-monophosphate; dUDP, 2′-deoxyurdine 5′-diphosphate; dUTP, 2′-deoxyurdine 5′-triphosphate