BRAFV600E mutational status assessment in cutaneous melanocytic neoplasms in a group of the Egyptian population

Table 2 Comparison of the BRAF^V600E mutational status by IHC and CAST-PCR in the 42 studied cases of melanocytic neoplasms

IHC results		BRAF^V600E CAST-PCR		Total	k-agreement p-value
IHC results		Mutant type	Wild type	Total	k-agreement p-value
Staining reaction	Positive	8 (a) 66.67%	8 (c) 26.7%	16 (38.1%)	0.364, p = 0.16
Staining reaction	Negative	4 (b) 33.3%	22 (d) 73.3%	26 (61.9%)	0.364, p = 0.16
Staining pattern	Homogenous	8 (a) (66.67%)	0 (c)	8	K = .0.741, p = .00*
Staining pattern	Heterogenous/absent	4 (b) (33.3%)	30 (d)	34	K = .0.741, p = .00*

PPV (sensitivity) = a/a + b, NPA (specificity) = c/c + d, Overall percent agreement (diagnostic accuracy) = a + d/total(n)
K kappa agreement coefficient, p probability
*significant

ISSN: 1475-2867