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Fig. 1 | Cancer Cell International

Fig. 1

From: Clinicopathological significance and prognostic role of LAG3 + tumor-infiltrating lymphocytes in colorectal cancer; relationship with sidedness

Fig. 1

Immunohistochemical staining pattern of CD3, CD8, LAG3, and CD45RO in colorectal cancer tissues. Center of tumor (CT) and Invasive margin (IM) regions. To create the best conditions for antigen retrieval and optimal dilution of primary antibodies, staining was first performed on human tonsil tissue with different dilutions of anti-CD3, anti-CD8, anti-CD45RO, and anti-LAG3 antibodies. The same method was performed with the same dilutions on CRC tissue and finally, 1/2.5 dilutions for CD3 marker, 1/150 for CD8 marker, 1/320 for CD45RO marker, and 1/1000 for LAG3 marker were determined as optimal dilutions. The figure shows the staining patterns of these markers with selected dilutions in different regions of CRC tissue

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Cancer Cell International

ISSN: 1475-2867

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