Fig. 4
Transplantation of A549-TIPARP cells induced angiogenesis in the CAM assay. a. Confirmation of the overexpression of TIPARP in A549 cells by semi-quantitative RT-PCR. GAPDH is used as a loading control. Water is used as a negative control. RT, reverse transcriptase. b. Macroscopic (upper panels) and microscopic (lower panels) images of CAM engrafted with A549-GFP cells and A549-TIPARP cells on EDD 15. A549-TIPARP cells induce angiogenesis on the CAM. Yellow dotted lines enveloped the mass. c. The vessel area (mm2) and mass area (mm2) were measured by Image J and are shown as dot plots in the left panel and right panel, respectively. The results were obtained from eight independent experiments. Mean values ± SE, unpaired t test **P < 0.01 and ***P < 0.001. d. Immunohistochemistry (IHC) to detect the Desmin expression of CAM engrafted with A549-GFP cells (left panels) and A549-TIPARP cells (right panels) on EDD 15 to stain chick vessels. Higher magnification images of the region outlined by yellow dotted lines in upper panels are shown in the lower panels. Red arrowheads indicate Desmin-positive chick vessels. Scale bars: 100 μm